The FlexiGene system uses simple centrifugation to isolate highly pure DNA from whole blood, buffy coat, and cultured cells. The FlexiGene DNA Kit contains buffers and QIAGEN Protease for direct, …

Pipet 200 µl whole blood, 20 µl QIAGEN Protease, and 200 µl Buffer AL into a 1.5 ml low binding microcentrifuge tube (e.g., Cat. No. T6050G, Marsh Biomedical Products, Rochester, NY).

Genomic DNA was purified from 2 ml (QIAamp Midi) or 10 ml (QIAamp Maxi) human whole blood and eluted in 300 µl (Midi) or 1 ml (Maxi) elution buffer. The first eluate was loaded onto the column a …

The total yield of genomic DNA from whole blood, buffy coat, and bone marrow samples depends on the sample volume and number of white blood cells/ml.

Each Maxwell®RSC Cartridge supplied in the Maxwell®RSC Buffy Coat DNA Kit is designed to purify genomic DNA from 50–250μl of buffy coat prepared from whole blood, assuming an average number …

Dilute peripheral blood 1 + 1, buffy coat 1 + 2, bone marrow 1+1 and umbilical cord blood 1 + 3 in PBS w/ 0.1% BSA + 0.6% Na-citrate or 2 mM EDTA. Layer up to 35 ml of the diluted sample over 15 ml …

2025年6月2日 · When you perform genomic DNA extraction from whole blood, low yield or low quality DNA can result in many issues. No matter your intended downstream application—qPCR, next …

The Sera-XtractaTM HMW DNA Kit is designed for the extraction and purification of genomic DNA from whole blood (treated with citrate, heparin or EDTA), buffy coat, saliva, cultured mammalian cells and …

The spin-column based Genomic DNA Isolation Kit (Blood/ Cultured Cell) was designed specifically for genomic DNA isolation from whole blood, frozen blood, buffy coat, cultured animal and bacterial …

Collect blood in EDTA-Na2 treated collection tubes (or other anticoagulant mixtures). Transfer up to 200 μl of blood or 200 μl of buffy coat to a sterile1.5 ml microcentrifuge tube.

Buffy coat DNA Introduction This protocol describes a method to extract high molecular weight genomic DNA from buffy coat, which has been isolated from rabbit blood, as an example of mammalian blood. …